PI: Donghun Shin
Title: Delineating the molecular mechanisms of hepatocyte-to-cholangiocyte reprogramming
PI: Sruti Shiva
Co-PI: Anthony J. Molina
Title: The relationship between blood based bioenergetics and muscle function, mobility, and aging
PI: Kang Kim
Title: Super Resolution Ultrasound Imaging of Vasa Vasorum to Characterize the Progression of Atherosclerotic Plaques and Predict Rupture Vulnerability
PI: Jonathan Vande Geest
Title: Preclinical Assessment of a Compliance Matched Biopolymer Vascular Graft
PIs: Pamela Moalli, Kyle Orwig, and Caroline Gargett
Title: Vaginal Stem Cells: The Missing Link in Vaginal Reconstruction
Program Director: James Luketich
Co-PDs: Julie Phillippi and David Vorp
Title: Cardiothoracic Surgery Research Training Program
PI: Andrew Duncan
Title: Mechanisms of Liver Regeneration Induced by Acetaminophen Toxicity
Description: Liver disorders affect 30 million people in the United States and are the country’s 10th leading cause of death. To improve liver disease treatments, a better understanding of hepatocyte biology is required. The liver contains diploid and polyploid hepatocytes, with polyploids comprising nearly 50% adult human and 90% adult mouse hepatocytes. The functional differences between diploid and polyploid hepatocytes are poorly understood. We previously demonstrated that diploid hepatocytes facilitate rapid liver regeneration, but it remains unknown how diploid and polyploid hepatocytes respond to drug-induced injury. Acetaminophen (APAP) is a common analgesic that can cause acute liver injury, resulting in liver failure and death when taken in excess. When APAP overdose causes extensive centrilobular hepatocyte death, the liver undergoes compensatory proliferation and recovers if the ratio of necrosis to regeneration is low. To investigate the role diploid and polyploid hepatocytes in vivo, we utilize E2f7 and E2f8 liver-specific knockout mice (LKO) where E2f7 and E2f8 are deleted during postnatal development; these mice are functionally normal through 6-9 months of age but are depleted of polyploid hepatocytes (LKO livers are >70% diploid). Preliminary studies indicate that LKO mice enriched with diploid hepatocytes are less damaged and recover faster from APAP injury than wild-type (WT) mice enriched with polyploid hepatocytes. The Grantee shall investigate mechanisms of liver injury and regeneration during APAP-induced liver injury and failure. This project shall determine ploidy-dependent and ploidy-independent mechanisms of liver repair and regeneration following APAP overdose.
PI: Alisse Hauspurg
Co-I: Ramakrishna Mukkamala, Sanjeev Shroff, and Aman Mahajan
Title: Development of a smartphone-based device to detect fluid overload among postpartum women with hypertensive disorders of pregnancy
PI: Mo Ebrahimkhani and Samira Kiani
Title: Collaborative Research: RECODE: Directed Differentiation of Human Liver Organoids via Computational Analysis and Engineering of Gene Regulatory Networks
PI: Kacey Marra
Title: Development of an Implantable Medical Device for Human Extremity Nerve Injuries
PI: William Wagner
Co-PI: John Alcorn, Stephen Badylak, Louis Falo, William Federspiel, Neeraj Gandhi, Eric Lagasse, Steven Little, Alan Wells, and Cecilia Yates
PI: William Federspiel / Ryan Orizondo
Title: Omniphobic coating of extracorporeal life support systems for improved thromboresistance (Subaward)
