Michael Behrens, a student in the lab of McGowan Institute for Regenerative Medicine affiliated faculty member Warren Ruder, PhD, associate professor and William Kepler Whiteford Faculty Fellow of Bioengineering at Pitt, created a side project to optimize his lab work. This side project piqued the interest of the global scientific community, putting it in the top 10 chemistry papers published in Scientific Reports in 2020.
Working as a bioengineering PhD student at Pitt’s Swanson School of Engineering, Mr. Behrens’ synthetic biology research at the University of Pittsburgh requires the use of microfluidic devices, which allow researchers to rapidly perform biology or chemistry experiments on a small scale.
Doing this work on a small scale helps save time and precious research dollars by allowing investigators to stretch resources, but Mr. Behrens saw more room for improvement. The peripheral equipment often required for microfluidic experiments adds to the cost and complexity, so he decided to innovate a solution to this problem.
Mr. Behrens’ open-source, 3D-printed tool is not only cheaper, but it also adds a level of flexibility for tech-savvy researchers to fully harness these transformative devices.
“Peristaltic pumps for microfluidic devices already exist, but I wanted a simple, reliable version that could carry small volumes of liquid,” said Mr. Behrens. “It’s cheaper to build it yourself, but we also added a level of flexibility by incorporating programmable microcontrollers that allow for custom flow profiles.”
Microfluidic devices have a wide range of applications, including point-of-care diagnostics – much like the testing we have witnessed for the COVID-19 pandemic. This type of technology, also known as lab-on-a-chip, can quickly deliver much needed results and has transformed testing – particularly in times of emergency, at-home care, or in places that lack clinical infrastructure.
“Since our pump is relatively cheap and easy to build and use, it could enable places with resource constraints to still have advanced diagnostics,” he said. “This pump could allow clinicians to run reagents past cells grown in a microfluidic device and do quick on-site testing or allow high school labs to experiment with modern chemistry and biology research techniques.”
For Mr. Behrens, the tool has helped advance his biorobotics research in the Synthetic Biology and Biomimetics Lab led by Dr. Ruder. He believes this tool could help fellow engineers stretch their budgets and more effectively utilize microfluidic technologies.
“I think there an unmet need to develop cheap tools to make microfluidics more accessible, especially for researchers,” he said. “The success of the paper shows me that a lot of people are interested in microfluidics and providing open-source tools to help enable those technologies seems like a useful thing to do.”
Congratulations, Mr. Behrens!
Illustration: Behrens (Ruder Lab)/Ruder (McGowan Institute for Regenerative Medicine)
Abstract (Open-source, 3D-printed peristaltic pumps for small volume point-of-care liquid handling. Michael R. Behrens, Haley C. Fuller, Emily R. Swist, Jingwen Wu, Md. Mydul Islam, Zhicheng Long, Warren C. Ruder & Robert Steward Jr. Scientific Reports, Volume 10, Article number: 1543 (2020).)