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Publication Of The Month | February 2011

    Home Publication of the Month Publication of the Month 2011 Publication Of The Month | February 2011

    Publication Of The Month | February 2011

    By The McGowan Institute For Regenerative Medicine | Publication of the Month 2011, Publication of the Month | 0 comment | 30 July, 2015 | 0

    Authors: Du Y, Roh DS, Funderburgh ML, Mann MM, Marra KG, Rubin JP, Li X, Funderburgh JL.

    Title: Adipose-derived stem cells differentiate to keratocytes in vitro.

    Summary: Adipose-derived stem cells (ADSC) are an abundant population of adult stem cells with the potential to differentiate into several specialized tissue types, including neural and neural crest-derived cells. This study sought to determine if ADSC express keratocyte-specific phenotypic markers when cultured under conditions inducing differentiation of corneal stromal stem cells to keratocytes.

    METHODS: Human subcutaneous adipose tissue was obtained by lipoaspiration. ADSC were isolated by collagenase digestion and differential centrifugation. Side population cells in ADSC were demonstrated using fluorescence-activated cell sorting after staining with Hoechst 33342. Differentiation to keratocyte phenotype was induced in fibrin gels or as pellet cultures with serum-free or reduced-serum media containing ascorbate. Keratocyte-specific gene expression was characterized using western blotting, quantitative RT-PCR, and immunostaining.

    RESULTS: ADSC contained a side population and exhibited differentiation to adipocytes and chondrocytes indicating adult stem-cell potential. Culture of ADSC in fibrin gels or as pellets in reduced-serum medium with ascorbate and insulin induced expression of keratocan, keratan sulfate, and aldehyde dehydrogenase 3 family, member A1 (ALDH3A1), products highly expressed by differentiated keratocytes. Expression of differentiation markers was quantitatively similar to corneal stromal stem cells and occurred in both serum-free and serum containing media.

    CONCLUSIONS: ADSC cultured under keratocyte-differentiation conditions express corneal-specific matrix components. Expression of these unique keratocyte products suggests that ADSC can adopt a keratocyte phenotype and therefore have potential for use in corneal cell therapy and tissue engineering.

    Source: Mol Vis. 2010 Dec 10;16:2680-9.

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      • Contact Us
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        • Cellular Therapies
        • Medical Devices and Artificial Organs
        • Clinical Translation
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      • Clinical Trials
      • Initiatives
    • Media
      • Current News
      • News Archive
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      • Wiegand Summer Internship
      • Admissions
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