Provided herein is a method for producing decellularized colonic extracellular matrix material. A decellularized colonic extracellular matrix material also is provided, along with uses for the material. Methods of use of the decellularized colonic extracellular matrix material also are provided, including methods of treating a defective, diseased, or damaged tissue or organ in a patient, and/or methods of treating esophageal disease, short bowel syndrome, ulcerative colitis, Crohn’s disease, or mucositis in a patient